Publications

Suleman, S., Fawaz, S., Roberts, T., Ellison, S., Bigger, B., Themis, M. (2024) ‘Optimised protocols to generate high titre lentiviral vectors using a novel transfection agent enabling extended HEK293T culture following transient transfection and suspension culture'. Journal of Virological Methods. https://doi.org/10.1016/j.jviromet.2024.114884

Suleman, S., Payne, A., Bowden, J., Al Haque, S., Zahn, M., Fawaz, S., Khalifa, M. S., Jobling, S., Hay, D., Franco, M., Fronza, R., Wang, W., Strobel-Freidekind, O., Deichmann, A., Takeuchi, Y., Waddington, S. N., Gil-Farina, I., Schmidt, M., Themis, M., (2022) ‘HIV- 1 lentivirus tethering to the genome is associated with transcription factor binding sites found in genes that favour virus survival’. Gene Therapy. 29. https://doi.org/10.1038/s41434-022-00335-4

Suleman, S., S chrubaji, K., Filippou, C., Ignatova, S., Hewitson, P., Huddleston, J., Karda, R., Waddington, S. N., Themis, M., (2021) 'Rapid and inexpensive purification of adenovirus vectors using an optimised aqueous two-phase technology'. Journal of Virological Methods. 229. https://doi.org/10.1016/j.jviromet.2021.114305

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Tijani, M., Munis, AM., Perry, C., Sanber, K., Ferraresso, M., Mukhopadhyay, T., et al. (2018) 'Lentivector Producer Cell Lines with Stably Expressed Vesiculovirus Envelopes'. Molecular Therapy - Methods and Clinical Development, 10. pp. 303 - 312. ISSN: 2329-0501

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Themis, M. (2018) 'Three-dimensional cell culture: From evolution to revolution'. Philos Trans R Soc Lond B Biol Sci, 373 (1750). pp. 20170216 - 20170216. ISSN: 0962-8436

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Khonsari, H., Schneider, M., Al-Mahdawi, S., Chianea, YG., Themis, M., Parris, C., et al. (2016) 'Lentivirus-meditated frataxin gene delivery reverses genome instability in Friedreich ataxia patient and mouse model fibroblasts'. Nature Gene Therapy, 23 (12). pp. 846 - 856. ISSN: 0969-7128

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Ghorbani, M., Themis, M. and Payne, AM. (2015) 'Genome wide classification and characterisation of CpG sites in cancer and normal cells'. Computers in Biology and Medicine, 68. pp. 57 - 66. ISSN: 0010-4825

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Condiotti, R., Goldenberg, D., Giladi, H., Schnitzer-Perlman, T., Simerzin, A., Galun, E., et al. (2014) 'Transduction of fetal mice with a feline lentiviral vector induces liver tumors which exhibit an E2F activation signature'. Molecular Therapy, 22 (1). pp. 59 - 68. ISSN: 1525-0016

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Nowrouzi, A., Arens, A., Paruzynski, A., Schmidt, M., Cheung, WT., Al-Allaf, F., et al. (2013) 'The fetal mouse is a sensitive genotoxicity model that exposes lentiviral-associated mutagenesis resulting in liver oncogenesis'. Molecular Therapy, 21 (2). pp. 324 - 337. ISSN: 1525-0016

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Themis, M. (2012) 'Monitoring for potential adverse effects of prenatal gene therapy: Genotoxicity analysis in vitro and on small animal models ex vivo and in vivo'.Methods in Molecular Biology, 891. pp. 341 - 370. ISSN: 1064-3745

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Coutelle, C., Waddington, SN. and Themis, M. (2012) 'Monitoring for potential adverse effects of prenatal gene therapy: Mouse models for developmental aberrations and inadvertent germ line transmission'. Methods in Molecular Biology, 891. pp. 329 - 340. ISSN: 1064-3745

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David, AL., Abi-Nader, KN., Weisz, B., Shaw, SWS., Themis, M., Cook, T., et al. (2010) 'ULTRASONOGRAPHIC DEVELOPMENT OF THE FETAL SHEEP STOMACH AND EVALUATION OF EARLY GESTATION ULTRASOUND-GUIDED IN UTERO I NTRAGASTRIC INJECTION'. TAIWANESE JOURNAL OF OBSTETRICS & GYNECOLOGY, 49 (1). pp. 23 - 29. ISSN: 1028-4559

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Kennea, NL., Waddington, SN., Chan, J., O'Donoghue, K., Yeung, D., Taylor, DL., et al. (2009) 'Differentiation of human fetal mesenchymal stem cells into cells with an oligodendrocyte phenotype'. Cell Cycle, 8 (7). pp. 1069 - 1079. ISSN: 1538-4101

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Buckley, SMK., Waddington, SN., Jezzard, S., Bergau, A., Themis, M., MacVinish, LJ., et al. (2008) 'Intra-amniotic delivery of CFTR-expressing adenovirus does not reverse cystic fibrosis phenotype in inbred CFTR-knockout mice'. Molecular Therapy, 16 (5). pp. 819 - 824. ISSN: 1525-0016

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David, AL., Weisz, B., Gregory, L., Themis, M., Cook, T., Roubliova, X., et al. (2006) 'Ultrasound-guided injection and occlusion of the trachea in fetal sheep'.Ultrasound in Obstetrics and Gynecology, 28 (1). pp. 82 - 88. ISSN: 0960-7692

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David, AL., Peebles, DM., Gregory, L., Waddington, SN., Themis, M., Weisz, B., et al. (2006) 'Clinically applicable procedure for gene delivery to fetal gut by ultrasound-guided gastric injection: Toward prenatal prevention of early-onset intestinal diseases'. Human Gene Therapy, 17 (7). pp. 767 - 779. ISSN: 1043-0342

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David, A., Weisz, B., Ruthe, A., Peebles, DM., Coutelle, C., Rodeck, CH. and et al. (2006) 'Gene transfer to human fetal tissues in vitro'. BJOG-AN INTERNATIONAL JOURNAL OF OBSTETRICS AND GYNAECOLOGY, 113 (7). pp. 863 - 863. ISSN: 1470-0328

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Themis, M., Waddington, SN., Schmidt, M., von Kalle, C., Wang, Y., Al-Allaf, F., et al. (2006) 'Erratum: "Oncogenesis following delivery of a nonprimate lentiviral gene therapy vector to fetal and neonatal mice" (Molecular Therapy (2005) vol. 12 (763-771) 10.1016/j.ymthe.2005.07.358)'. Molecular Therapy, 13 (4). pp. 830. ISSN: 1525-0016

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Themis, M., Waddington, SN., Schmidt, M., von Kalle, C., Wang, Y., Al-Allaf, F., et al. (2006) 'Oncogenesis following delivery of a nonprimate lentiviral gene therapy vector to fetal and neonatal mice (vol 12, pg 763, 2005)'. Molecular Therapy, 13 (4). pp. 830 - 830. ISSN: 1525-0024

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Tolmachov, O., Ma, YL., Themis, M., Patel, P., Spohr, H., Macleod, KT., et al. (2006) 'Overexpression of connexin 43 using a retroviral vector improves electrical coupling of skeletal myoblasts with cardiac myocytes in vitro'. BMC Cardiovascular Disorders, 6 (1). pp. 25 - 25. ISSN: 1471-2261

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Bigger, BW., Siapati, EK., Mistry, A., Waddington, SN., Nivsarkar, MS., Jacobs, L., et al. (2006) 'Permanent partial phenotypic correction and tolerance in a mouse model of hemophilia B by stem cell gene delivery of human factor IX.'. Gene Therapy, 13 (2). pp. 117 - 126. ISSN: 0969-7128

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Coutelle, C., Themis, M., Waddington, SN., Buckley, SMK., Gregory, LG., Nivsarkar, MS., et al. (2005) 'Gene therapy progress and prospects: Fetal gene therapy - First proofs of concept - Some adverse effects'. Gene Therapy, 12 (22). pp. 1601 - 1607. ISSN: 0969-7128

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Themis, M., Waddington, SN., Schmidt, M., von Kalle, C., Wang, YH., Al-Allaf, F., et al. (2005) 'Oncogenesis following delivery of a non-primate lentiviral gene therapy vector to fetal mice'.Autumn Meeting of the British-Toxicology-Society. Univ Bath, Bath, ENGLAND. 19 - 20 September. ELSEVIER IRELAND LTD. pp. 233 - 233. ISSN: 0300-483X

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Siapati, EK., Bigger, BW., Miskin, J., Chipchase, D., Parsley, KL., Mitrophanous, K., et al. (2005) 'Comparison of HIV- and EIAV-based vectors on their efficiency in transducing murine and human hematopoietic repopulating cells'. Molecular Therapy, 12 (3). pp. 537 - 546. ISSN: 1525-0016

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Weisz, B., David, AL., Gregory, LG., Perocheau, D., Ruthe, A., Waddington, SN., et al. (2005) 'Targeting the respiratory muscles of fetal sheep for prenatal gene therapy for Duchenne muscular dystrophy'. American Journal of Obstetrics and Gynecology, 193 (3). pp. 1105 - 1109. ISSN: 1097-6868

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Buckley, SM., Waddington, SN., Jezzard, S., Lawrence, L., Schneider, H., Holder, MV., et al. (2005) 'Factors influencing adenovirus-mediated airway transduction in fetal mice'. Molecular Therapy, 12 (3). pp. 484 - 492. ISSN: 1525-0016

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Al-Allaf, FA., Tolmachov, O., Themis, M. and Coutelle, C. (2005) 'Coupled analysis of bacterial transformants and ligation mixture by duplex PCR enables detection of fatal instability of a nascent recombinant plasmid'. Journal of Biochemical and Biophysical Methods, 64 (2). pp. 142 - 146. ISSN: 0165-022X

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Bigger, BW., Waddington, SN., Jones, N., Buch, M., Jacobs, L., Perrett, R., et al. (2005) 'Non-myeloablative stem cell gene delivery effectively establishes long-term gene expression and mixed chimerism in immunocompetent mice'.34th Annual Meeting of the International-Society-for-Experimental-Hematology. Univ Glasgow, Glasgow, SCOTLAND. 30 - 2 August. ELSEVIER SCIENCE INC. pp. 41 - 42. ISSN: 0301-472X

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Waddington, SN., Kramer, MG., Hernandez-Alcoceba, R., Buckley, SM., Themis, M., Coutelle, C. and et al. (2005) 'In utero gene therapy: Current challenges and perspectives'. Molecular Therapy, 11 (5). pp. 661 - 676. ISSN: 1525-0016

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Al-Allaf, F., Tolmachov, O., Themis, M. and Coutelle, C. (2005) 'Accurate size gauging of ExoIII/S1-generated deletions by PCR analysis of ligation mixtures'. Anal Biochem, 339 (2). pp. 348 - 350. ISSN: 0003-2697

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Themis, M., Bennett, PR., Mehmet, H., Waddington, SN. and Buckley, SM. (2005) 'The development of a mouse model to study the effect of uterine infection on preterm labour and fetal outcome.'.52nd Annual Meeting of the Society-for-Gynecologic-Investigation. Los Angeles, CA. 23 - 26 March. ELSEVIER SCIENCE INC. pp. 259A - 259A. ISSN: 1071-5576

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Weisz, B., David, A., Peebles, D., Gregory, L., Perocheau, D., Waddington, S., et al. (2005) 'Targeting the fetal respiratory muscles for prenatal gene therapy for Duchenne muscular dystrophy'.25th Annual Meeting of the Society-for-Maternal-Fetal-Medicine. Reno, NV. 7 - 12 February. MOSBY-ELSEVIER. pp. S22 - S22.ISSN: 0002-9378

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David, A., Weisz, B., Peebles, D., Roubliova, X., Perocheau, D., Themis, M., et al. (2005) 'Ultrasound guided placement of an occlusive balloon in the trachea of fetal sheep'.25th Annual Meeting of the Society-for-Maternal-Fetal-Medicine. Reno, NV. 7 - 12 February. MOSBY-ELSEVIER. pp. S166 - S166. ISSN: 0002-9378

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Themis, M., Waddington, SN., Schmidt, M., von Kalle, C., Wang, Y., Al-Allaf, F., et al. (2005) 'Oncogenesis following delivery of a nonprimate lentiviral gene therapy vector to fetal and neonatal mice.'. Molecular Therapy, 12 (4). pp. 763 - 771. ISSN: 1525-0016

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Waddington, SN., Nivsarkar, MS., Mistry, AR., Buckley, SMK., Kemball-Cook, G., Mosley, KL., et al. (2004) 'Permanent phenotypic correction of hemophilia B in immunocompetent mice by prenatal gene therapy'. BLOOD, 104 (9). pp. 2714 - 2721. ISSN: 0006-4971

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Gregory, LG., Waddington, SN., Holder, MV., Mitrophanous, KA., Buckley, SM., Mosley, KL., et al. (2004) 'Highly efficient EIAV-mediated in utero gene transfer and expression in the major muscle groups affected by Duchenne muscular dystrophy'. Gene Therapy, 11 (14). pp. 1117 - 1125. ISSN: 0969-7128

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Themis, M., Gregory, LG., Waddington, SN., Holder, MV., Mitrophanous, KA., Buckley, SMK., et al. (2004) 'The fetal approach: A novel therapy for the treatment of musculo-skeletal disease'.7th Annual Meeting of the American-Society-of-Gene-Therapy. Minneapolis, MN. 2 - 6 June. ACADEMIC PRESS INC ELSEVIER SCIENCE. pp. S91 - S91. ISSN: 1525-0016

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Bigger, BW., Siapati, EK., Mistry, A., Waddington, SN., Nivsarkar, MS., Holder, MV., et al. (2004) 'Induction of immune tolerance and phenotypic rescue of hemophilic mice by stem cell mediated gene transfer'.7th Annual Meeting of the American-Society-of-Gene-Therapy. Minneapolis, MN. 2 - 6 June. ACADEMIC PRESS INC ELSEVIER SCIENCE. pp. S64 - S64. ISSN: 1525-0016

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Ma, YI., Tolmachov, O., Themis, M., Spohr, H., Kienast, Y., Coutelle, C. and et al. (2004) 'Field action potential activation and propagation in cocultured cardiac myocytes and Cx43 transduced skeletal myoblasts in a multi-electrode array'.Annual Conference of the British-Cardiac-Society. Manchester, ENGLAND. 24 - 27 May. B M J PUBLISHING GROUP. pp. A37 - A37. ISSN: 1355-6037

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Waddington, SN., Buckley, SM., Bernloehr, C., Bossow, S., Ungerechts, G., Cook, T., et al. (2004) 'Reduced toxicity of F-deficient Sendai virus vector in the mouse fetus'. Gene Ther, 11 (7). pp. 599 - 608. ISSN: 0969-7128

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Bergau, A., Gregory, L., Waddington, S., Harbottle, R., Lawrence, L., Coutelle, C. and et al. (2004) 'Comparative analysis of the human cytokeratin K18 promoter with the CMV promoter by hydrodynamic injection of adult MF-1 mice'.1st Annual Conference of the British-Society-for-Gene-Therapy. Keble Coll, Oxford, ENGLAND. 28 - 30 March. JOHN WILEY & SONS LTD. pp. S5 - S6. ISSN: 1099-498X

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Bigger, BW., Siapati, KE., Mistry, A., Waddington, SN., Nivsarkar, MS., Holder, MV., et al. (2004) 'Induction of immune tolerance and partial phenotypic correction of haermophiliac mice by lentiviral gene transfer of human factor IX to haematopoietic stem cells'.1st Annual Conference of the British-Society-for-Gene-Therapy. Keble Coll, Oxford, ENGLAND. 28 - 30 March. JOHN WILEY & SONS LTD. pp. S15 - S15. ISSN: 1099-498X

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Holder, MV., Waddington, SN., Gregory, LG., Kramer, MG., Prieto, J., Mitrophanous, K., et al. (2004) 'Comparison of gene expression from liver specific promoter/enhancer combinations in the context of a lentiviral backbone'.1st Annual Conference of the British-Society-for-Gene-Therapy. Keble Coll, Oxford, ENGLAND. 28 - 30 March. JOHN WILEY & SONS LTD. pp. S9 - S9. ISSN: 1099-498X

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Gregory, LG., Waddington, SN., Holder, M., Mitrophanous, KA., Buckley, SMK., Ellard, FM., et al. (2004) 'Pseudotyped lentiviral vectors: Toward an applicability for in utero treatment of Duchenne Muscular Dystrophy'.1st Annual Conference of the British-Society-for-Gene-Therapy. Keble Coll, Oxford, ENGLAND. 28 - 30 March. JOHN WILEY & SONS LTD. pp. S18 - S18. ISSN: 1099-498X

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Chan, J., O'Donoghue, K., Kennea, N., de la Fuente, J., Waddington, S., Themis, M., et al. (2004) 'Human fetal mesenchymal stem cells for intrauterine cellular therapy'.1st Annual Conference of the British-Society-for-Gene-Therapy. Keble Coll, Oxford, ENGLAND. 28 - 30 March. JOHN WILEY & SONS LTD. pp. S17 - S17.ISSN: 1099-498X

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Themis, M. (2004) 'An in vitro hprt locus insertion mutagenesis assay'.1st Annual Conference of the British-Society-for-Gene-Therapy. Keble Coll, Oxford, ENGLAND. 28 - 30 March. JOHN WILEY & SONS LTD. pp. S37 - S37. ISSN: 1099-498X

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Waddington, SN., Nivsarkar, M., Mistry, A., Buckley, SMK., Kemball-Cook, G., Mosley, KL., et al. (2004) 'Permanent phenotypic correction of Haemophilia B in immunocompetent mice by prenatal gene therapy'.1st Annual Conference of the British-Society-for-Gene-Therapy. Keble Coll, Oxford, ENGLAND. 28 - 30 March. WILEY-BLACKWELL. pp. S17 - S17. ISSN: 1099-498X

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Nivsarkar, MS., Nathwani, A., Mosley, K., Davies, N., Gregory, LG., David, AL., et al. (2004) 'Testosterone induced increase in transgene expression in vivo using Adenoviral Gene Delivery Vectors'.1st Annual Conference of the British-Society-for-Gene-Therapy. Keble Coll, Oxford, ENGLAND. 28 - 30 March. WILEY-BLACKWELL. pp. S12 - S13. ISSN: 1099-498X

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Weisz, B., David, AL., Peebles, DM., Gregory, L., Perocheau, D., Themis, M., et al. (2004) 'Targeting the fetal gut for prenatal gene therapy.'.51st Annual Meeting of the Society-for-Gynecologic-Investigation. Houston, TX. 24 - 27 March. ELSEVIER SCIENCE INC. pp. 182A - 182A. ISSN: 1071-5576

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Alison, MR., Vig, P., Russo, F., Bigger, BW., Amofah, E., Themis, M. and et al. (2004) 'Hepatic stem cells: From inside and outside the liver?'. Cell Proliferation, 37 (1). pp. 1 - 21. ISSN: 0960-7722

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Waddington, SN., Buckley, SMK., Gregory, LG., Themis, M., Coutelle, C. and Kennea, NL. (2004) 'Fetal and neonatal gene therapy: Benefits and pitfalls'.2nd European Conference and Practical Course on Towards Clinical Gene Therapy - Preclinical Gene Transfer Assessment. Univ Autonoma Barcelona, Fac Vet, Bellaterra, SPAIN. 1 - 14 February. Springer Science and Business Media LLC. pp. S92 - S97. ISSN: 0969-7128

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Mellor, N., Themis, M., Selden, C., Jones, M. and Hodgson, HJF. (2004) 'Characteristics of murine histidinaemia and its potential for genetic manipulation'. Liver International, 24 (4). pp. 354 - 360. ISSN: 1478-3223

Peebles, D., Gregory, LG., David, A., Themis, M., Waddington, SN., Knapton, HJ., et al. (2004) 'Widespread and efficient marker gene expression in the airway epithelia of fetal sheep after minimally invasive tracheal application of recombinant adenovirus in utero'. Gene Therapy, 11 (1). pp. 70 - 78. ISSN: 0969-7128

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Themis, M., May, D., Coutelle, C. and Newbold, RF. (2003) 'Mutational effects of retrovirus insertion on the genome of V79 cells by an attenuated retrovirus vector: Implications for gene therapy'. Gene Therapy, 10 (19). pp. 1703 - 1711. ISSN: 0969-7128

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David, AL., Peebles, DM., Gregory, L., Themis, M., Cook, T., Coutelle, C. and et al. (2003) 'Percutaneous ultrasound-guided injection of the trachea in fetal sheep: A novel technique to target the fetal airways'. Fetal Diagnosis and Therapy, 18 (5). pp. 385 - 390. ISSN: 1015-3837

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Waddington, SN., Mitrophanous, KA., Ellard, FM., Buckley, SM., Nivsarkar, M., Lawrence, L., et al. (2003) 'Long-term transgene expression by administration of a lentivirus-based vector to the fetal circulation of immuno-competent mice'. Gene Therapy, 10 (15). pp. 1234 - 1240. ISSN: 0969-7128

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Azzouz, M., Le, TN., Waddington, S., Walmsley, L., Monani, U., Wilkes, FJ., et al. (2003) 'Lentiviral vector-mediated gene therapy for a mouse model of spinal muscular atrophy'.6th Annual Meeting of the American-Society-of-Gene-Therapy. WASHINGTON, D.C. 4 - 8 June. ACADEMIC PRESS INC ELSEVIER SCIENCE. pp. S248 - S248. ISSN: 1525-0016

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Themis, M., Waddington, S., Holder, M., Buckley, S., Mitrophanos, K., Bigger, B., et al. (2003) 'Highly efficient and comprehensive gene transfer to all muscle groups necessary for the potential treatment of Duchenne/Becker muscular dystrophies following in utero delivery of a lentivirus vector in immuno-competent mice'.6th Annual Meeting of the American-Society-of-Gene-Therapy. WASHINGTON, D.C. 4 - 8 June. ACADEMIC PRESS INC ELSEVIER SCIENCE. pp. S320 - S320. ISSN: 1525-0016

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Gregory, LG., Peebles, D., David, A., Themis, M., Waddington, SN., Knapton, HJ., et al. (2003) 'Widespread and efficient adenovirus-mediated gene transfer to the airways by DEAE dextran and sodium caprate in adult mice and fetal sheep after minimally invasive tracheal application'.6th Annual Meeting of the American-Society-of-Gene-Therapy. WASHINGTON, D.C. 4 - 8 June. NATURE PUBLISHING GROUP. pp. S142 - S143. ISSN: 1525-0016

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Buckley, SMK., Waddington, SN., Jezzard, S., Themis, M., Colledge, WH. and Coutelle, C. (2003) 'Intra-amniotic application of CFTR-expressing adenovirus does not reverse cystic fibrosis phenotype in inbred Cftr-knockout mice'.6th Annual Meeting of the American-Society-of-Gene-Therapy. WASHINGTON, D.C. 4 - 8 June. ACADEMIC PRESS INC ELSEVIER SCIENCE. pp. S200 - S200. ISSN: 1525-0016

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Waddington, SN., Buckley, SMK., Cook, T., Gregory, L., Rahim, A., Nivsarkar, M., et al. (2003) 'Fetal gene delivery using first and second generation Sendai virus'.6th Annual Meeting of the American-Society-of-Gene-Therapy. WASHINGTON, D.C. 4 - 8 June. ACADEMIC PRESS INC ELSEVIER SCIENCE. pp. S4 - S4. ISSN: 1525-0016

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David, A., Cook, T., Waddington, S., Peebles, D., Nivsarkar, M., Knapton, H., et al. (2003) 'Ultrasound-guided percutaneous delivery of adenoviral vectors encoding the β-galactosidase and human factor IX genes to early gestation fetal sheep In Utero'. Human Gene Therapy, 14 (4). pp. 353 - 364. ISSN: 1043-0342

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Waddington, SN., Buckley, SMK., Nivsarkar, M., Jezzard, S., Schneider, H., Dahse, T., et al. (2003) 'In utero gene transfer of human factor IX to fetal mice can induce postnatal tolerance of the exogenous clotting factor'. BLOOD, 101 (4). pp. 1359 - 1366. ISSN: 0006-4971

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Coutelle, C., Themis, M., Waddington, S., Gregory, L., Nivsarkar, M., Buckley, S., et al. (2003) 'The hopes and fears of in utero gene therapy for genetic disease - A review'.Symposium on From Genetics to Implantation and Early Fetal Development in Infertile Couples. MILAN, ITALY. 1 - 25 October. Elsevier BV. pp. S114 - S121.ISSN: 0143-4004

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Grogory, LG., Harbotte, RP., Lawrence, L., Knapton, HJ., Themis, M. and Coutelle, C. (2003) 'Enhancement of adenovirus-mediated gene transfer to the airways by DEAE dextran and sodium caprate in vivo'. Molecular Therapy, 7 (1). pp. 19 - 26. ISSN: 1525-0016

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Selden, C., Casbard, A., Themis, M. and Hodgson, HJF. (2003) 'Characterization of long-term survival of syngeneic hepatocytes in rat peritoneum'. Cell Transplantation, 12 (6). pp. 569 - 578. ISSN: 1555-3892

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Ma, YL., Tolmachov, O., Themis, M., Poznansky, M., Coutelle, C. and Peters, NS. (2002) 'Enhanced gap-junctional coupling in connexin43 transduced skeletal myoblasts'.American-Heart-Association Abstracts From Scientific Sessions. CHICAGO, ILLINOIS. 17 - 20 November. LIPPINCOTT WILLIAMS & WILKINS. pp. 281 - 281. ISSN: 0009-7322

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Ma, Y., Tolmacho, O., Themis, M., Poznansky, M., Coutelle, C. and Peters, NS. (2002) 'Intercellular communication in connexin43 transfected skeletal myocytes'.Congress of the European-Society-of-Cardiology. BERLIN, GERMANY. 31 - 4 September. W B SAUNDERS CO LTD. pp. 42 - 42. ISSN: 0195-668X

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Eden, ER., Patel, DD., Sun, XM., Burden, JJ., Themis, M., Edwards, M., et al. (2002) 'Restoration of LDL receptor function in cells from patients with autosomal recessive hypercholesterolemia by retroviral expression of ARH1'. Journal of Clinical Investigation, 110 (11). pp. 1695 - 1702. ISSN: 0021-9738

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Tolmachov, O., Themis, M., Poznansky, M., Coutelle, C. and Peters, N. (2001) 'Construction of connexin 43 transducing retrovirus to produce electrocompetent skeletal muscle tissue for transplantation into the damaged heart'. European Heart Journal, 22. pp. 429 - 429. ISSN: 1522-9645

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Chan, LMC., Coutelle, C. and Themis, M. (2001) 'A novel human suspension culture packaging cell line for production of high-titre retroviral vectors'. Gene Therapy, 8 (9). pp. 697 - 703. ISSN: 0969-7128

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Coutelle, C., Themis, M., Schneider, H., Kiserud, T., Cook, T., Douar, AM., et al. (2001) 'Fetal somatic gene therapy--a preventive approach to the treatment of genetic disease: the case for.'.33rd Ernst-Schering-Research-Foundation Workshop. BERLIN, GERMANY. 1 Springer Berlin Heidelberg. pp. 99 - 114. ISSN: 0947-6075

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Mellor, N., Themis, M., Selden, C., Jones, M. and Hodgson, HJ. (2000) 'Amelioration of murine histidinaemia using retroviral gene transfer'. Hepatology, 32 (4). pp. 182A - 182A. ISSN: 1527-3350

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Schneider, H., Groves, M., Mühle, C., Reynolds, PN., Knight, A., Themis, M., et al. (2000) 'Retargeting of adenoviral vectors to neurons using the Hc fragment of tetanus toxin'. Gene Therapy, 7 (18). pp. 1584 - 1592. ISSN: 0969-7128

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Forbes, SJ., Themis, M., Alison, MR., Shiota, A., Kobayashi, T., Coutelle, C. and et al. (2000) 'Tri-iodothyronine and a deleted form of hepatocyte growth factor act synergistically to enhance liver proliferation and enable in vivo retroviral gene transfer via the peripheral venous system'. Gene Therapy, 7 (9). pp. 784 - 789. ISSN: 0969-7128

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McKay, TR., MacVinish, LJ., Carpenter, B., Themis, M., Jezzard, S., Goldin, R., et al. (2000) 'Selective in vivo transfection of murine biliary epithelia using polycation-enhanced adenovirus'. Gene Therapy, 7 (8). pp. 644 - 652. ISSN: 0969-7128

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Forbes, SJ., Themis, M., Alison, MR., Sarosi, I., Coutelle, C. and Hodgson, HJ. (2000) 'Synergistic growth factors enhance rat liver proliferation and enable retroviral gene transfer via a peripheral vein'. Gastroenterology, 118 (3). pp. 591 - 598. ISSN: 0016-5085

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Mellor, N., Themis, M., Selden, C. and Hodgson, HJ. (1999) 'An in-vitro model of histidinemia ameliorated by a histidase encoding retrovirus'. Hepatology, 30 (4). pp. 514A - 514A. ISSN: 1527-3350

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Forbes, SJ., Fajinmi, MA., Themis, M., Alison, MR., Coutelle, C. and Hodgson, HH. (1999) 'Gene transfer to the carbon tetrachloride damaged rat liver in vivo with adenoviral and retroviral based vectors'. Hepatology, 30 (4). pp. 514A - 514A. ISSN: 1527-3350

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Schneider, H., Adebakin, S., Themis, M., Cook, T., Douar, AM., Pavirani, A. and et al. (1999) 'Therapeutic Plasma Concentrations of Human Factor IX in Mice after Gene Delivery into the Amniotic Cavity: A Model for the Prenatal Treatment of Haemophilia B'. Journal of Gene Medicine, 1 (6). pp. 424 - 432. ISSN: 1099-498X

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Themis, M., Schneider, H., Kiserud, T., Cook, T., Adebakin, S., Jezzard, S., et al. (1999) 'Successful expression of β-galactosidase and factor IX transgenes in fetal and neonatal sheep after ultrasound-guided percutaneous adenovirus vector administration into the umbilical vein'. Gene Therapy, 6 (7). pp. 1239 - 1248. ISSN: 0969-7128

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Themis, M., Forbes, SJ., Chan, L., Cooper, RG., Etheridge, CJ., Miller, AD., et al. (1998) 'Enhanced in vitro and in vivo gene delivery using cationic agent complexed retrovirus vectors'. Gene Therapy, 5 (9). pp. 1180 - 1186. ISSN: 0969-7128

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Forbes, SJ., Themis, M., Alison, MR., Selden, C., Coutelle, C. and Hodgson, HJF. (1998) 'Retroviral gene transfer to the liver in vivo during tri-iodothyronine induced hyperplasia'. Gene Therapy, 5 (4). pp. 552 - 555. ISSN: 0969-7128

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Douar, AM., Adebakin, S., Themis, M., Pavirani, A., Cook, T. and Coutelle, C. (1997) 'Foetal gene delivery in mice by intra-amniotic administration of retroviral producer cells and adenovirus'. Gene Therapy, 4 (9). pp. 883 - 890. ISSN: 0969-7128

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Douar, AM., Themis, M., Sandig, V., Friedmann, T. and Coutelle, C. (1996) 'Effect of amniotic fluid on cationic lipid mediated transfection and retroviral infection'.Gene Therapy, 3 (9). pp. 789 - 796. ISSN: 0969-7128

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Douar, AM., Themis, M. and Coutelle, C. (1996) 'Fetal somatic gene therapy'. Molecular Human Reproduction, 2 (9). pp. 633 - 641. ISSN: 1360-9947

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Trott, DA., Cuthbert, AP., Todd, CM., Themis, M. and Newbold, RF. (1995) 'Novel use of a selectable fusion gene as an "in-out" marker for studying genetic loss in mammalian cells'. Molecular Carcinogenesis, 12 (4). pp. 213 - 224. ISSN: 0899-1987

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Cuthbert, AP., Trott, DA., Ekong, RM., Jezzard, S., England, NL., Themis, M., et al. (1995) 'Construction and characterization of a highly stable human:Rodent monochromosomal hybrid panel for genetic complementation and genome mapping studies'. Cytogenetic and Genome Research, 71 (1). pp. 68 - 76. ISSN: 1424-8581

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